Effect of growth regulators on direct Miscanthus × giganteus organogenesis in vitro

Abstract

Research was carried out at the Institute of Biology and Plant Biotechnology of Aleksandras Stulginskis University and at the Laboratory of Agrobiotechnology of the Joint Research Centre. The effect of growth regulators on the direct organogenesis from isolated explants of Miscanthus × giganteus was analysed. Rhizome segments of Miscanthus × giganteus were cultivated in the Murashige and Skoog (MS) nutrient medium supplemented with different 6-benzylaminopurine (BAP) or thidiazuron (TDZ) and ɑ-naftilacetic acid (NAA) combinations. It has been determined that the isolated explants in the nutrient medium without growth regulators did not form adventitious shoots. In the nutrient medium supplemented with growth regulators the regeneration frequency varied from 5.6 to 21.1%. The adventitious shoots formation frequency due to the TDZ exposure, depending on the concentration, was from 5.4 to 10.0% higher (in combination with 0.05 mg l^–1 NAA) and from 4.4 to 8.8% higher (in combination with 0.1 mg l^–1 NAA) in comparison with the adequate concentration of TDZ. The most intensive shoot formation frequency and the highest shoot number per explant have been obtained in the medium supplemented with 2.0 mg l^–1 TDZ + 0.05 mg l^–1 NAA.