In 2012, Lithuanian scientists and society at large celebrated the 60th anniversary of the Laboratory of Parasitology and the 130th birth anniversary of its founder academician of the Lithuanian Academy of Sciences professor P. B.Šivickis, who launched traditions of broad basic parasitology research in Lithuania. This paper describes main achievements of the current staff of the P. B. Šivickis Laboratory of Parasitology at Nature Research Centre (Vilnius) in basic parasitology studies, particularly molecular and morphological characterization of eukaryotic parasites.