Signalling of indole-3-acetic acid: inhibitory analysis of MAP kinase / phosphatase pathways

Anotacija

The aim of this work was to study the involvement of MAP kinase / phosphatase pathways in the IAA signal transduction of intensively elongating plant cells by treatment with inhibitors of these pathways and use plants with a disturbed signal transduction cascade. The results obtained by application of inhibitors such as staurasporine, H8, sodium metavanadate, dideoxyadenosine and activator of membrane-associated protein kinase C – phorbol ester – support the idea of the transduction of IAA-induced signal into the nucleus via the MAP kinase / phosphatase signalling cascade. Hypocotyls of Arabidopsis thaliana mpk6 knockout lines showed inhibited signal transduction in comparison to WT ones. These data corroborate the idea that MAP kinase modules, at least partially, participate in IAA signal transduction together with the other signalling pathways. Keywords: IAA, inhibitors and activators of signal transduction, wheat coleoptiles, thale cress hypocotyls