Study of ssDNA immobilization and hybridization on gold substrate with quartz crystal microbalance

  • J. Kupčiūnaitė
  • A. Kaušaitė
  • A. Ramanavičius
  • A. Ramanavičienė

Anotacija

The aim of this study was to apply QCM for the registration of synthetic ssDNA fragments binding to SAM and for DNA hybridization. 5’-aminoalkyl- oligonucleotides were successfully covalently immobilized onto SAM /Au via carboxylate ester linkages, and we observed a reduction in the frequency of 91 Hz. For duplex formation, the oligo-50mer after incubation with 10× ligation buffer was placed on an ssDNA-modified surface, and we observed a reduction in the frequency of 112 Hz. It means that covalently we immobilized 629 ng/cm2 of 5’-amino-alkyl-oligonucleotides. After the duplex formation, the gold-coated quartz mass increased by 776 ng/cm2. Keywords: QCM, ssDNA, DNA sensor
Publikuotas
2006-07-01
Skyrius
Molecular Biology