Retention dependence on temperature in reversed-phase and hydrophilic interaction liquid chromatography

Anotacija

The retention mechanism in reversed-phase liquid chromatography (RPLC) and in hydrophilic interaction liquid chromatography (HILIC) have been investigated by examining the temperature dependence of retention of the chosen phenolic acids on two columns modified by hydrosilated silica – hydrosilated silica column, Diamond hydride column, cholesterol column and C18 bidentate column. The linear dependences of retention on temperature were observed in the HILIC as well as in the RPLC on all columns tested. Van’t Hoff equation was applied to describe the retention mechanism thoroughly, and its slopes and intercepts were used for calculations of thermodynamic constants for the transfer of a solute from the mobile phase to the stationary phase (ΔH0 and ΔS0). Keywords: phenolic acids, HILIC, van’t Hoff equation, hydrosilated silica