Silver ion solid-phase extraction for the analysis of trans fatty acids in human adipose

Abstract

Silver ion solid-phase extraction is suggested for the analysis of trans fatty acids in human adipose. Acid catalyzed esterification of the fatty acids protocol using boron trifluoride–methanol complex as a catalyst was used. Silica based Ag+-SPE conditions are presented. A mixture of dichloromethane and hexane with the content of dichloromethane increasing from 5 to 100% was used as an eluent. The presented method allows eliminating from the trans chromatographic zone about 75% of cis isomers of fatty acid methyl esters, thus enabling to determine trace contents of trans fatty acids in the samples containing big amounts of dominant saturated, monounsaturated and polyunsaturated fatty acids.