The comparative study of redox properties of recombinant human cytosolic and mitochondrial NADPH: thioredoxin reductases

  • Lina Misevičienė
  • Kastis Krikštopaitis
  • Narimantas Čėnas
Keywords: thioredoxin reductase, redox potential, xenobiotics, bioreductive activation


We compared the redox properties of recombinant cytosolic (TrxR1) and mitochondrial (TrxR2) isoforms of human flavosulfoselenoenzyme NADPH:thioredoxin reductase. The standard redox potentials of isoenzymes (E0 7), determined according to the redox equilibrium with the NADP+/NADPH couple, were equal to –0.295 V (TrxR1) and –0.270 V (TrxR2). The more positive value of E0 7 of TrxR2 may be attributed to the presence of His-125 at the vicinity of catalytic disulfide and selenylsulfide, instead of Tyr-116 in TrxR1. The reactivity of several quinones and nitroaromatic compounds towards TrxR1 and TrxR2 increased with their single-electron reduction potential (E1 7). For the first time, we studied the TrxR1-catalysed reduction of a series of aromatic N-oxides which were reduced in a mixed single- and two-electron way. Their reactivity was close to that of quinones and nitroaromatics with the similar values of E1 7.

Organic Chemistry