https://lmaleidykla.lt/ojs/index.php/biologija/issue/feed Biologija 2025-10-01T11:14:39+03:00 Editorial Assistant biologija@vdu.lt Open Journal Systems <p><em>Biologija</em>&nbsp;covers a broad spectrum of themes and brings together such various disciplines as general biology, microbiology, parasitology, molecular biology and ecology, genetics, mathematical modelling, biochemistry and biotechnology and others. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for a deeper understanding of the effects of natural processes and human behaviour/activities on environment and the anthropogenic impact of economic development on ecosystems.</p> https://lmaleidykla.lt/ojs/index.php/biologija/article/view/6516 Title 2025-10-01T10:00:41+03:00 Lietuvos mokslų akademija ojs@lmaleidyba.lt <p>&nbsp;&nbsp;</p> 2025-09-30T00:00:00+03:00 Copyright (c) https://lmaleidykla.lt/ojs/index.php/biologija/article/view/6519 Contents 2025-10-01T10:06:40+03:00 Lietuvos mokslų akademija ojs@lmaleidyba.lt <p>&nbsp;&nbsp;</p> 2025-09-30T00:00:00+03:00 Copyright (c) https://lmaleidykla.lt/ojs/index.php/biologija/article/view/6520 Wheat performance is affected by the substitution of chemical fertilisers with animal manure under different soil moisture regimes 2025-10-01T11:14:39+03:00 Ismael Noori Jumaah mjanmohammadi@maragheh.ac.ir Mohsen Janmohammadi mjanmohammadi@maragheh.ac.ir Amin Abbasi mjanmohammadi@maragheh.ac.ir Asghar Ebadi-Segherloo mjanmohammadi@maragheh.ac.ir Naser Sabaghnia mjanmohammadi@maragheh.ac.ir <p>The intensification of the&nbsp;effects of climate change and the&nbsp;necessity of using climate-resilient methods and improving nutritional conditions, replacing chemical fertilisers with organic fertilisers and saving water consumption should be heightened in semi-arid regions. A&nbsp;field experiment was conducted to evaluate the&nbsp;effect of different doses of NPK chemical fertilisers (CH<sub>0</sub>: no use of NPK fertiliser, CH<sub>50</sub>: application of 50% of the&nbsp;recommended dose of NPK, CH<sub>100</sub>: application of 100% of the&nbsp;recommended dose of NPK) and different levels of animal manure (0, 5, and 10&nbsp;t&nbsp;ha<sup>–1</sup>: FYM<sub>0</sub>, FYM<sub>5</sub> and FYM<sub>10</sub>) under different soil moisture conditions (full irrigation (FI) and rainfed with one supplementary irrigation at the&nbsp;spike stage (SI)) on winter wheat performance in Hamedan area. Under FI conditions, the&nbsp;lateral growth of the&nbsp;canopy increased strongly with the&nbsp;use of chemical fertilisers. However, the&nbsp;longitudinal growth and internode distances increased with the&nbsp;combined application of chemical and organic fertilisers. Under SI, the&nbsp;application of FYM<sub>10</sub>&nbsp;+&nbsp;CH<sub>50</sub> had a&nbsp;greater effect on yield components. Grain weight decreased under both irrigation regimes with the&nbsp;increase in the&nbsp;consumption of NPK fertilisers. The&nbsp;highest grain yield (4790&nbsp;kg&nbsp;ha<sup>–1</sup>) was obtained for plants grown under FI&nbsp;+&nbsp;FYM<sub>10</sub>&nbsp;+&nbsp;CH<sub>100</sub> and plants grown with FYM<sub>10</sub>&nbsp;+&nbsp;CH<sub>50</sub> (4425&nbsp;ha<sup>–1</sup>). Under the&nbsp;SI conditions, the&nbsp;highest yield was recorded with the&nbsp;utilization of FYM<sub>10</sub>&nbsp;+&nbsp;CH<sub>50</sub> (2644), and FYM<sub>10</sub>&nbsp;+&nbsp;CH<sub>0</sub> (2549), and the&nbsp;lowest yield was recorded under FYM<sub>0</sub>. Taken together, the&nbsp;use of chemical fertilisers under FI conditions is inevitable to increase the&nbsp;grain yield, and the&nbsp;replacement of NPK fertiliser with the&nbsp;current applied amounts of animal fertilisers could not be adequate to improve the&nbsp;grain yield considerably.</p> 2025-09-30T00:00:00+03:00 Copyright (c) https://lmaleidykla.lt/ojs/index.php/biologija/article/view/6521 Trace metal distribution in Tilapia zillii and Synodontis schall from the Siluko River, Edo State, Nigeria 2025-10-01T11:14:26+03:00 Egun Nkonyeasua Kingsley kingsley.egun@uniben.edu Olowo Ukpamufo Cyril kingsley.egun@uniben.edu Biose Ekene kingsley.egun@uniben.edu Jacob N. Jacob kingsley.egun@uniben.edu Oboh Ijeoma Patience kingsley.egun@uniben.edu <p>Metal pollution of aquatic ecosystems has raised concerns about their uptake by aquatic organisms and subsequent biomagnification along the&nbsp;aquatic food chain. This study determined trace metal distribution in <em>Tilapia zillii</em> and <em>Synodontis schall</em> from the&nbsp;Siluko River in Edo State, Nigeria. Fresh fish samples were harvested monthly from September 2021 to August 2022 with the&nbsp;assistance of artisanal fishermen. Trace metal contents in the&nbsp;gills, liver, and muscles were determined using atomic absorption spectrophotometer. The&nbsp;metal pollution index (MPI) was used to estimate the&nbsp;extent of metal bioaccumulation in the&nbsp;fishes. Results showed species variation in metal accumulation in various organs:<em> T.&nbsp;zillii</em> had a&nbsp;higher mean content of Cu, Zn, Mn and Fe in all the&nbsp;organs, while <em>S.&nbsp;schall</em> had a&nbsp;higher mean content of Ni, Cr, and Pb in all the&nbsp;organs. The&nbsp;MPI values ranged from 5.28 to 5.94 in the&nbsp;organs of <em>T.&nbsp;zillii</em> and <em>S.&nbsp;schall</em>. The&nbsp;order of MPI values in<em> T.&nbsp;zillii</em> was muscles &gt; liver &gt; gills; in <em>S.&nbsp;schall</em>, it was liver &gt; gills &gt; muscles. The&nbsp;results indicate trace metal contamination of fishes due to aquatic pollution. High MPI values is an indication of the&nbsp;degree of metal pollution of the&nbsp;Siluko River and the&nbsp;high susceptibility of these metals to bioaccumulation in the&nbsp;fishes. This predisposes the&nbsp;fishes to metal toxicity and portends health risk to fish consumers. The&nbsp;continuous monitoring of activities within the&nbsp;river watershed to mitigate heavy metal pollution is recommended.</p> 2025-09-30T00:00:00+03:00 Copyright (c) https://lmaleidykla.lt/ojs/index.php/biologija/article/view/6522 Assessment of immunity to the measles virus through the detection of IgG antibodies 2025-10-01T11:14:13+03:00 Indrė Lipatova indre.lipatova@vdu.lt Justina Dagytė indre.lipatova@vdu.lt Algimantas Paulauskas indre.lipatova@vdu.lt <p>Measles is a&nbsp;highly contagious viral infection that can lead to severe complications. The&nbsp;aim of this study was to evaluate immunity of the&nbsp;target population against the&nbsp;measles virus by assessing the&nbsp;presence of IgG antibodies. A&nbsp;total of 176 individuals were tested using the&nbsp;ELISA method. Fourteen participants (7.95%) were found to be non-immune, primarily from the&nbsp;1976–1981 and 1982–1987 birth cohorts. The&nbsp;highest proportion of seronegative individuals (17.4%) was observed among those with unknown vaccination status. No significant differences were found between sexes. Most participants were from Kaunas, which also accounted for the&nbsp;majority of seronegative cases.</p> 2025-09-30T00:00:00+03:00 Copyright (c) https://lmaleidykla.lt/ojs/index.php/biologija/article/view/6523 Antioxidant effects of quercetin on Chinese hamster ovary (CHO) cells and their electroporation 2025-10-01T11:14:01+03:00 Laura Kairytė raminta.rodaite@vdu.lt Indrė Ivanauskienė raminta.rodaite@vdu.lt Raminta Rodaitė raminta.rodaite@vdu.lt <p>Electroporation is a&nbsp;phenomenon when a&nbsp;brief application of a&nbsp;strong electric field affects biological membranes, temporarily increasing their permeability to ions and molecules. As a&nbsp;result, transient pores are created in the&nbsp;plasma membrane. During this process, electrochemical reactions occur both at the&nbsp;electrode-solution interface and within the&nbsp;medium, leading to the&nbsp;generation of reactive oxygen species (ROS) that can impair cell viability. In this study, we investigated the&nbsp;effects of ROS induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and electroporation on Chinese hamster ovary (CHO) cells, as well as the&nbsp;potential antioxidant protection provided by quercetin. CHO cell suspensions were exposed to various concentrations of H<sub>2</sub>O<sub>2</sub> and quercetin and electroporated using a single 2 ms duration electric field pulse of varying intensities (0.5–2.0&nbsp;kV/cm). Cell viability was evaluated using a&nbsp;colony formation assay, and ROS levels were assessed via Amplex Red fluorescence. Quercetin showed no cytotoxicity up to 2&nbsp;µM, but reduced cell viability at higher concentrations. It significantly improved survival in cells exposed to H<sub>2</sub>O<sub>2</sub> across all tested concentrations, with the&nbsp;most notable effect observed at 10&nbsp;µM H<sub>2</sub>O<sub>2</sub>, increasing viability. Similarly, quercetin enhanced post-electroporation viability by 11% and 17% at 0.5 and 1.0&nbsp;kV/cm, respectively. However, at 2.0&nbsp;kV/cm, its protective effect was no longer observed. These results suggest that quercetin mitigates oxidative stress induced by both chemical and physical stimuli and may be a&nbsp;useful cytoprotective agent in electroporation-based biomedical applications.</p> 2025-09-30T00:00:00+03:00 Copyright (c) https://lmaleidykla.lt/ojs/index.php/biologija/article/view/6524 Abstracts of the BalTick25: The Baltic Tick Conference 2025-10-01T11:01:02+03:00 Lietuvos mokslų akademija ojs@lmaleidyba.lt <p>&nbsp;&nbsp;</p> 2025-09-30T00:00:00+03:00 Copyright (c)