Stable DNA–protein complexes in yeast Saccharomyces cerevisiae. Protein composition analysis

Abstract

Proteins tightly bound to DNA (TBP) are a protein group that remains attached to DNA by covalent or non-covalent bonds after its deproteinisation [1, 2]. These proteins are released only after DNA digestion with DNase I or benzonase. TBP have been found in DNA of numerous evolutionary distant species [3, 4]. Only a few of these TBP have been characterized. In the present work, we have identified some proteins that are present in Saccharomyces cerevisiae TBP–DNA protein complexes by two-dimensional electrophoresis and MALDI-TOF MS analysis. Most of these proteins (chromatin assembly factor 1, NNF1 protein, DNA damage tolerance protein RHC31, DNA repair protein RAD 7, SOH1 protein, etc.) have been found to participate in chromatin rearengament and regulation processes. Keywords: DNA–protein interaction, stable DNA–protein complexes