Self-sufficient cytochrome P450 monooxygenases from Bacillus thuringiensis: amplification and comparative sequence analysis

  • Romualdas GRIŠKEVIČIUS
  • Nomeda KUISIENĖ
  • Juozas RAUGALAS
  • Džiuginta JASINSKYTĖ
  • Donaldas ČITAVIČIUS


Four primers targeting the self-sufficient cytochrome P450 monooxygenase gene in the Bacillus cereus group were designed in this study. PCR was carried out using these primers, and Bacillus thuringiensis wild type strains C32 and 1.2 as well as the reference strain HER1410 were proven to possess this gene. Our results also showed that B. thuringiensis C32 possesses two different copies of this gene. Phylogenetic analysis and comparative sequence analysis were performed in order to evaluate the differences between the sequences of B. thuringiensis C32, 1.2 and HER1410 as well as those from the public databases. The sequence of B. thuringiensis HER1410 clustered with that of B. cereus 14579T, and the sequences of B. thuringiensis C32 and 1.2 clustered with that of Bacillus weihenstephanensis KBAB4. The sequences of both B. thuringiensis Al Hakam and B. thuringiensis serovar konkukian 97–27 did not cluster with strains C32, 1.2 and HER1410. The diversity of CYP102A in B. thuringiensis strains implies that this species represents an excellent potential resource of different self-sufficient cytochrome P450 monooxygenases. Keywords: cytochrome P450, self-sufficient cytochrome P450 monooxygenase, Bacillus thuringiensis, Bacillus cereus group, Bacillus megaterium, CYP102A