The effect of photodynamic treatment on the activity of regulatory glycolytic enzymes in A431 cells

Abstract

The effect of photodynamic treatment on the activity of regulatory glycolytic enzymes hexokinase (EC 2.7.1.1) and pyruvate kinase (EC 2.7.1.40) has been investigated. The experiments were carried out on human epidermoid carcinoma A431 cells photosensitised with either safranin O (Safr) or mTHPC. An increased concentration of ATP in the treated cells has been observed. The Safr-mediated treatment at CD80 and mTHPC-mediated treatment at CD50 induced an immediate sustained significant reduction of hexokinase and pyruvate kinase activities. No changes were found in the activities of both enzymes when cells were treated with Safr at CD50. The activities of HK and PK were reduced by PDT under conditions involving damage to mitochondrial outer membrane. Keywords: photodynamic treatment, photosensitisation, ATP, A431 cells, hexokinase, piruvate kinase