Pseudomonas mendocina 3121-1 lipase-catalysed lipolysis. The effect of detergents

Abstract

The role of various detergents in the lipolysis of olive oil emulsion catalyzed by Pseudomonas mendocina 3121-1 lipase was investigated. Sodium dodecylsulphate (SDS) was determined to inactivate the lipolysis of emulsified substrate, whereas dodecyltrimethylammonium bromide (DTMAB) activated the process independently of the addition moment, and sodium deoxycholate (SDCh) strongly activated the lipolysis when added after the reaction had been started. The effect of Tweens on the lipolysis showed their synergetic character as compared to the lipolysis of emulsified substrates only and to the hydrolysis of non-ionic detergents. The effect was shown to be different from that of Triton X-100. The effect of guanidine hydrochloride (Gdn-HCl) on the lipolysis was found to be of different character as compared with the hydrolysis of the soluble substrate. Keywords: bacterial lipase, detergents, lipolysis, substrate specificity