Effect of Polyscias filicifolia Bailey tincture on tRNALeu and leucyl-tRNA synthetase activity in isolated pig heart

Abstract

The aim of the present study was to investigate the effect of Polyscias filicifolia Bailey tincture on the activity of tRNA specific for leucine (tRNALeu) and leucyl-tRNA synthetase under anoxia in isolated pig heart. Polyscias filicifolia Bailey tincture did not affect the acceptor activity of tRNALeu from a control pig heart, but after 20 min of anoxic perfusion with a buffer containing Polyscias filicifolia Bailey tincture the activity of organic pyrophosphatase was the same as in control. It has been shown that Polyscias filicifolia Bailey protects tRNA molecules from conformational changes. Polyscias filicifolia Bailey tincture did not change activity of leucyl-tRNA synthetase in norm. After 20 min of anoxic perfusion with a buffer containing Polyscias filicifolia Bailey tincture, activity of this enzyme reached the control value, whereas after 90 min of anoxic perfusion with the same buffer activity of leucyl-tRNA synthetase it did not completely restore and reached 77% of the control value. Changes in the activity of leucyl-tRNA synthetase correlated with the changes in the activity of inorganic pyrophosphatase. It has been supposed that Polyscias filicifolia Bailey tincture protects the activity of leucyl-tRNA synthetase through the protective action on inorganic pyrophosphatase. Our results have shown that Polyscias filicifolia Bailey biomass tincture restores activity of translation machinery components under anoxia. Keywords: anoxia, pig heart, Polyscias filicifolia Bailey, tRNA, leucyl-tRNA synthetase