Pseudomonas mendocina 3121-1 lipase-catalysed reaction of oleic acid with glycerol

Abstract

The specificity of Pseudomonas mendocina 3121-1 lipase for oleic acid esterification with glycerol was investigated. The examination of esterification of oleic acid showed that the most effective conditions for this reaction were obtained at the 1:7 molar ratio of fatty acid (FA) to glycerol at a temperature of 30 0C. By thin layer chromatography (TLC) it has been determined that 2-methyl-2-propanol (tert-butanol) is not a substrate for Ps. mendocina (PmL) 3121-1 lipase, because there was no ester formation. Keywords: bacterial lipase, ensymatic esterification, thin layerchromatography