Characterization of kolomikta kiwi (Actinidia kolomikta) genetic diversity by RAPD fingerprinting

Abstract

Twenty-four accessions of Actinidia kolomikta (Maxim.) Maxim. were evaluated by the RAPD method at the Kaunas Botanical Garden collection ex situ for genetic diversity. Six decamer oligonucleotides generated 43 fragments, of which 30 (69.8%) were polymorphic. The UPGMA dendrogram revealed a wide range of genetic variability and a relationship between the accessions. Three fragments were detected in all genotypes. The cultivar ‘Laiba’ showed the highest GDxy values and was selected as a genetic distinct accession in the A. kolomikta germplasm collection. Keywords: DNA, genetic relationship, polymorphism, RAPD