Dot-immunoperoxidase assay for the demonstration of soluble Fcg receptors

Abstract

We have developed a sensitive dot-immunoperoxidase assay to demonstrante and characterize the functional activity of soluble Fcγ receptors (sFcγR). Samples containing sFcγR were immobilized on nitrocellulose membrane. Immune complexes of horseradish peroxidase (HRP) and rabbit IgG antibodies to HRP were visualized with an HRP developer. Dot-immunoperoxidase assay is described as a rapid and simple method for the demonstration and characterization of functionally active sFcγR. Keywords: soluble Fcγ receptor, immune complex, dot-immunoperoxidase assay INTRODUCTION Several dot-immunobinding assays have been described [1, 2], and the techniques have been useful in the characterization of autoantigens, allergens and infectious organis