Design of a chitosan-based bio-scaffolding for regenerative medicine: compatibility analysis for cartilage regeneration

Seyed Ali (Behruz) Khaghani, Gunay Akbarova, Gulrukh Dilbazi


Background. Evaluation of any material that is utilised for clinical applications is essential in order to ensure that it acquires physical, mechanical and biocompatible properties required for its proposed function. Understanding of the conditions that regulate the mechanical and physical characteristics of a material particularly biopolymers is crucial in the field of cell and tissue engineering. The aim of this work is to assemble some polymers and crosslink with each other using β-glycerophosphate to produce a scaffolding which is suitable for in vitro tissue regeneration.
Materials and methods. The experimental procedures in this project involved the culturing of cells in different suspensions containing control, CSG hydrogel, fibronectin, and hydrogel with fibronectin. The aim of the conducted experiment was to find the cell viability and cytotoxicity analysis of CSG hydrogel, and to study how the cells interact with CSG hydrogel as a possible candidate for articular cartilage repair. Another aim of this work was to find out whether the cells can grow and survive in CSG hydrogel for a period of 24 hours.
Results and conclusions. The results obtained from series of tests proved that CSG3 has the most optimum properties, as it presented the highest viscosity at 37°C, the highest stability during temperature changes, and an enhanced elastic behaviour at the physiological temperature.


Chitosan; hydrogel; β-glycerophosphate; crosslinking; cell culture

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ISSN 1392-0146 (Print)
ISSN 2029-0578 (Online)